A solution containing Aspartic acid (pI=2.98), Glycine - TopicsExpress

A solution containing Aspartic acid (pI=2.98), Glycine (pI=5.97), Threonine (pI=6.53) and Lysine (pI=9.74) in 50 mM citrate buffer pH 3.0 was applied to a cation exchange column equilibrated with the same buffer and fraction collected. The order of elution of these amino acids from the column is (1) Lysine, Threonine, Leucine, Glycine, Aspartic acid (2) Asprtic acid, Threonine, Glycine, Leucine, Lysine (3) Aspartic acid, Glycine, Leucine, Threonine, Lysine (4) Aspartic acid, Threonine, Leucine, Glycine, Lysine

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