A student is asked to isolate and purify various subcellular organelles from a liver cell sample. Following sucrose density gradient centrifugation technique, and taking utmost care mitochondrial fraction is found to be contaminated with nuclear fraction. Which technique out of the choices given below should be appropriate to obtain a clean mitochondrial fraction? A. Gel filtration B. Percoll density gradient C. Immuno‐magnetic separation D. Pulse field electrophoresis
Posted on: Sat, 19 Apr 2014 11:14:52 +0000
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