Biochemistry No. Theory classes: 70hours No. of practical classes: 20 hours Theory: Specimen collection: Pre-analytical variables, Collection of blood, Collection of CSF & other fluids Urine collection, Use of preservatives, Anticoagulants • Introduction to Laboratory apparatus o Pipettes- different types (Graduated, volumetric, Pasteur, Automatic etc.,) o Calibration of glass pipettes o Burettes, Beakers, Petri dishes, depression plates. o Flasks - different types )Volumetric, round bottomed, Erlemeyer conical etc.,) o Funnels – different types (Conical, Buchner etc.,) o Bottles – Reagent bottles – graduated and common, Wash bottles – different type Specimen bottles etc., • Measuring cylinders, Porcelain dish o Tubes – Test tubes, centrifuge tubes, test tube draining rack o Tripod stand, Wire gauze, Bunsen burner. o Cuvettes, significance of cuvettes in colorimeter, cuvettes for visible and UV range, cuvette holders Racks – Bottle, Test tube, Pipette o Dessicator, Stop watch, rimers, scissors o Dispensers – reagent and sample o Any other apparatus which is important and may have been missed should also be covered o Maintenance of lab glass ware and apparatus: o Glass and plastic ware in Laboratory o *use of glass: significance of boro silicate glass; care and cleaning of glass ware, different cleaning solutions of glass o * care and cleaning of plastic ware, different cleaning solution • Instruments (Theory and demonstration) Diagrams to be drawn o Water bath: Use, care and maintenance o Oven & Incubators : Use, care and maintenance. o Water Distillation plant and water deionisers. Use, care and maintenance o Refrigerators, cold box, deep freezers – Use, care and maintenance o Reflux condenser : Use, care and maintenance o Centrifuges (Theory and demonstration) Diagrams to be drawn o Definition, Principle, svedberg unit, centrifugal force, centrifugal field rpm, ref. Conversion of G to rpm and vice versa. o Different types of centrifuges o Use care and maintenance of a centrifuge o Laboratory balances (Theory & Practicals) Diagrams to be drawn o Manual balances: Single pan, double pan, trip balance o Direct read out electrical balances. o Use care and maintenance. Guideline to be followed and precautions to be taken while weighing o Weighing different types of chemicals, liquids. Hygroscopic compounds etc. o Colorimeter and spectrophotometer (Theory and Practicals) Diagrams to be drawn Principle, Parts Diagram. o Use, care and maintenance. o pH meter (Theory & practicals) Diagrams to be drawn o principle, parts, Types of electrodes, salt bridge solution. o Use, care and maintenance of Ph meter and electrodes o Guidelines to be followed and precautions to be taken while using pH meter • Safety of measurements • Conventional and SI units • Atomic structure o Dalton’s theory, Properties of electrons, protons, neutrons, and nucleus, Rutherford’s model of atomic structure, Bohr’s model of atomic structure, orbit and orbital, Quantum numbers, Heisenberg’s uncertainly principle. o Electronic configuration – Aufbau principle, Pauli’s exclusion principle, etc. Valency and bonds – different types of strong and weak bonds in detail with examples o Theory & Practicals for all the following under this section Molecular weight, equivalent weight of elements and compounds, normality, molarity Preparation of molar solutions (mole/litre solution) eg: 1 M Nacl, 0.15 M NaCL 1 M NaOH, 0.1 M HCl, 0.1 M H 2S04 etc., Preparation of normal solutions. eg., IN Na2CO3, O IN Oxalic acid, 0.1 N HCl, 0.1N H2504, 0.66 N H2S04 etc., Percent solutions. Preparation of different solutions – v/v w/v (solids, liquids and acids) Conversion of a percent solution into a molar solution • Dilutions
Posted on: Thu, 19 Sep 2013 13:26:56 +0000