EMERGING OPTION FOR ENDING PAIN IN THE BACK, KNEE JOINT, ETC BY REBUILDING THE TISSUE WITH STERILE AMNIOTIC TISSUE. READ ALL THE REPAIR CHEMICALS HERE. THIS IS NOT YOUR MOMMYS MOTRIN. THIS IS ONLY KNOWN BY AGGRESSIVE AND INTENSELY MOTIVATED PAIN DOCS. THIS CAN REPLACE A JOINT REPLACEMENT IF IT WORKS! **Amniotic tissues contain a matrix of collagen (a connective tissue found in the body) that can serve as scaffolding for the body to build new tissues around. Additionally, amniotic tissue contains a high concentration of stem cells. These cells are particularly useful because they do not contain the markers found in more mature cells that cause body reactions when an incompatible donor is used. Amniotic tissue also contains other growth factors involved in tissue growth. Due to this combination of beneficial properties, amniotic tissues have received considerable interest as a potential therapy for treating conditions such as joint degeneration. Research on this therapy is still early, but promising so far. In one study, researchers found that amniotic membrane was capable of creating growth in cartilage from joints. THANKS DR. T. SWING SOURCE: ONE IS PLANNED C-SECTIONS WITH SCREENED MOTHERS. BTW KILLING BABESIA DROPS VEGF WHICH HELPS WOUNDS REPAIR. AND IT IS NO SURPRISE SOME WITH TICK INFECTIONS HAVE SCRATCHES THAT TAKE 4 MONTHS TO HEAL. STUDY BELOW WITH ANOTHER SOURCE. ALREADY IN USE IN USA AND EVEN GOOD RESULTS IN NAPLES HERE FOR REAL PAIN. Angiogenic properties of dehydrated human amnion/chorion allografts: therapeutic potential for soft tissue repair and regeneration Thomas J Koob1*, Jeremy J Lim1, Michelle Massee1, Nicole Zabek1, Robert Rennert2, Geoffrey Gurtner2 and William W Li3 Abstract Background: Chronic wounds are associated with a number of deficiencies in critical wound healing processes, including growth factor signaling and neovascularization. Human-derived placental tissues are rich in regenerative cytokines and have been shown in randomized clinical trials to be effective for healing chronic wounds. In this study, PURION® Processed (MiMedx Group, Marietta, GA) dehydrated human amnion/chorion membrane tissue allografts (dHACM, EpiFix®, MiMedx) were evaluated for properties to support wound angiogenesis.Methods: Angiogenic growth factors were identified in dHACM tissues using enzyme-linked immunosorbent assays (ELISAs), and the effects of dHACM extract on human microvascular endothelial cell (HMVEC) proliferation and production of angiogenic growth factors was determined in vitro. Chemotactic migration of human umbilical vein endothelial cells (HUVECs) toward pieces of dHACM tissue was determined using a standard in vitro transwell assay. Neovascularization of dHACM in vivo was determined utilizing a murine subcutaneous implant model.Results: Quantifiable levels of the angiogenic cytokines angiogenin, angiopoietin-2 (ANG-2), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), heparin binding epidermal growth factor (HB-EGF), hepatocyte growth factor (HGF), platelet derived growth factor BB (PDGF-BB), placental growth factor (PlGF), and vascular endothelial growth factor (VEGF) were measured in dHACM. Soluble cues promoted HMVEC proliferation in vitro and increased endogenous production of over 30 angiogenic factors by HMVECs, including granulocyte macrophage colony-stimulating factor (GM-CSF), angiogenin, transforming growth factor β3 (TGF-β3), and HB-EGF. 6.0 mm disks of dHACM tissue were also found to recruit migration of HUVECs in vitro. Moreover, subcutaneous dHACM implants displayed a steady increase in microvessels over a period of 4 weeks, indicative of a dynamic intra-implant neovascular process. CONCLUSIONS: Taken together, these results demonstrate that dHACM grafts: 1) contain angiogenic growth factors retaining biological activity; 2) promote amplification of angiogenic cues by inducing endothelial cell proliferation and migration and by upregulating production of endogenous angiogenic growth factors by endothelial cells; 3) support the formation of blood vessels in vivo. dHACM grafts are a promising wound care therapy with the potential to promote revascularization and tissue healing within poorly vascularized, non-healing wounds. Keywords: Amnion, Chorion, Amnion/chorion grafts, dHACM, Angiogenesis, Growth factors, VEGF, Endothelial cells, Soft tissue regeneration, Wound healing, Chronic wounds * Correspondence: tkoob@mimedx 1MiMedx Group, Inc., 1775 West Oak Commons Ct., Marietta, GA, USA Koob et al. Vascular Cell 2014, 6:10 vascularcell/content/6/1/10 Background Normal wound healing is a complex biological process requiring interactions among distinct resident cell types, as well as inflammatory cells, platelets, and stem cells. Growth of new blood vessels into the wound through angiogenesis is a critical aspect of this process, to promote the adequate delivery of nutrients and regulatory factors required for tissue remodeling and regeneration.
Posted on: Fri, 02 Jan 2015 01:26:28 +0000
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