Emory and CDC no doubt based on prior TX research that can no longer be ignored....... Characterization of the Bacterial Communities of Life Stages of Free Living Lone Star Ticks (Amblyomma americanum). ncbi.nlm.nih.gov/pubmed/25054227 PLoS One. 2014 Jul 23;9(7):e102130. doi: 10.1371/journal.pone.0102130. eCollection 2014. Williams-Newkirk AJ1, Rowe LA2, Mixson-Hayden TR3, Dasch GA4. Author information Abstract The lone star tick (Amblyomma americanum) is an abundant and aggressive biter of humans, domestic animals, and wildlife in the southeastern-central USA and an important vector of several known and suspected zoonotic bacterial pathogens. However, the biological drivers of bacterial community variation in this tick are still poorly defined. Knowing the community context in which tick-borne bacterial pathogens exist and evolve is required to fully understand the ecology and immunobiology of the ticks and to design effective public health and veterinary interventions. We performed a metagenomic survey of the bacterial communities of questing A. americanum and tested 131 individuals (66 nymphs, 24 males, and 41 females) from five sites in three states. Pyrosequencing was performed with barcoded eubacterial primers targeting variable 16S rRNA gene regions 5-3. The bacterial communities were dominated by Rickettsia (likely R. amblyommii) and an obligate Coxiella symbiont, together accounting for 6.7-100% of sequences per tick. DNAs from Midichloria, Borrelia, Wolbachia, Ehrlichia, Pseudomonas, or unidentified Bacillales, Enterobacteriaceae, or Rhizobiales groups were also detected frequently. Wolbachia and Midichloria significantly co-occurred in Georgia (p
Posted on: Thu, 24 Jul 2014 23:33:38 +0000
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