PAN Oligo Synthesis: Error rates for nanopore discrimination among - TopicsExpress

PAN Oligo Synthesis: Error rates for nanopore discrimination among cytosine, methylcytosine, and hydroxymethylcytosine along individual DNA strands. -- Modification of cytosine bases in DNA can determine when genes are turned on in biological cells. These modifications are important during cell differentiation, embryogenesis, and aberrant cell growth in cancer... Oligonucleotides were purchased from the Stanford Peptide and Nucleic Acid Facility... The phosphoramidite for the abasic residue was dideoxyribose-3′-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite. FormC, the phosphoramiditewas 5′-dimethoxytrityl-N-benzoyl-5-methyl-2′-deoxycytidine-3′-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite...

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