The complete digestion of DNA fragment using a specific - TopicsExpress

The complete digestion of DNA fragment using a specific restriction enzyme will produce an average fragment size of about 22kb. When the restricted fragments are separated using 0.8% agarose gel, a single band was seen. The reason could be a. The DNA was contaminated with protein b. Too much DNA was loaded on the gel c. Problem in the pH of the buffer d. Agarose will not separate DNA fragment above 20kb

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