The preparing of the cancer cells for apoptosis: 1.the activation of the MAPK pathway by the cascades activations of the MEK proteins and the ERK1,2 proteins which activates the genes a. MYC,MYB for the cellular growth . b. FOS ,JUN, for induces the apoptosis . 2. the over activities of the protein TP53 which activates the proteins BAX,NOXA,PUMA which activates the apoptosis. 3.the mutations in the RB1 genes or loss of it help for over activation for the E2F1 which activate the ways for the apoptosis . 4.the RAS protein which acts as a switch between many oncogenes pathways which activates the NORE1, RASSIA which leads for the apoptosis. 5.The anti apoptosis proteins : a. BCL2( its type BCL-W) inactivates the TP53. b. BCL-XL (its type MCL-1,A1,NRF3) activates the NFkB. 6. the pro-apoptotic proteins : a. BAX protein (its type BAK)activate TP53,and (its type BOK ) inactivates the AKT protein . b. the BID protein activates the caspasis 8, and the PUMA activates the TP53 ,and the NIX protein regulates the hypoxia 7.the mechanisms which disrupt the apoptosis in the cancer cells play an important roles for these cells survivals : a. mutations in the death receptors in the extrinsic pathway. b. mutations or disruptions in the genes which form the proteins for the intrinsic pathway . c loss or defect in the genes TP53. d. over expression of the protein IAPs. Activation for the anti-apoptotic pathways . From these important notes we can prepare the cancer cells for the apoptosis by the next steps: 1. we take normal tissues of the same type of the cancer cells and we excite in it the normal pathways for the cellular death by: a. excite the death receptors for the normal tissues ( out of the body) by its specific growth factors. b. by formation the immune complexes ( antigens with the anti bodies with the complements ( different types ) as in the researches before to destroy these normal cells by the apoptosis pathways . 2. we isolate the excited proteins from the residues of the death cells ,because these proteins contain multi types of the apoptotic proteins with low types of the anti-apoptotic proteins ,to use it later as( switch proteins ) . 3. in the cancer cells with the inappropriate proliferations and differentiations which excite the genes of the anti-apoptotic proteins like the BCL2 families , so we find high concentrations of these proteins in the cancer cells( due for many divisions) while we find it with low concentrations in the normal cells . 4.the most important notes in the process of the apoptosis: a. the important roles of the P53 protein in the inducing the BAX ,NOXA,PUMA in the mitochondria and the BAX is the antagonist for the BCL2 in the intrinsic pathway ,so the P53 protein help us for the apoptosis . b. the importance of the death recptors FAS/CD95 , DR4/DR5, DR3, TNF and its adaptors the FAAD and the TRADD and the activators which bind the of the death ligands like the FASL/CD95 , TRAIL/APO-2L ,APO-3L , TF ,so the uses especially of the TRAIL ( which acts on the death receptors the TRAIL-R1 and the TRAIL-R2 ) proteins with the high toxicities on the normal cells especially the hepatocytes ,so the uses of the TNF which secreted from the macrophages and the monocytes can activate the TNF-R1 receptor and induce the apoptosis ( the immune complexes which mediates the IL1 and the IL2). c .as the RAS play an important actions for the connections between many oncogenes pathways and activates the NORE1 and the most important RASSF1A protein (which form from the 3p21,3 chromosome and binds to the microtubules which block the mitosis ,and the activation of the apoptosis by reverse the actions of the AKT proteins which prevents the BAD protein to attach the mitochondrial proteins and prevents also the FKHR-L1( which activates the apoptosis) to enters the nucleus ,so the uses of the P53 , the RASSF1A, FKHR-L1 proteins in our researches and attack the AKT proteins with the antibodies ( or the isotopes ) can we change the directions from the activations of the cellular divisions from the oncogenes pathways for activations the different mechanisms of the apoptosis in the same times ( the synergistic effects ) 5.we use the researches before like : a. The complete weakness for the cancer cells in the part II. b. The new treatments for aids and cancer and genes deformities by the mutated promoters in the part II. c. The theoretic research on the cellular immunity in the part I. 5. after exciting for the killer cells and make the weakness in the cancer cells we direct these cells for the apoptosis by using the switch proteins because it contains high doses of the apoptotic proteins ,to reverse and neutralize the anti-apoptotic proteins ( part of the switch proteins ) and the ratio between the apoptotic and anti –apoptotic proteins directs the cancer cells for the apoptosis ,and because the reverse actions of the anti-apoptotic proteins by the switch proteins with the high concentrations ,helping for activations also the E2F1,and the oncogenes pathways for activations the FOS ,JUN genes to complete the apoptosis ,and to facilitate the actions of the killer cells . The important notes : 1. the immune complex needs the antigens ,antibodies ,and the complement system ,but in many times it needs the super antigen to evoke the immune response, due to complete the proteins balances ( electromagnetic balances ,due to the different charges on the protein surfaces , attractive or incongruity )by filling the empty gaps in the immune complexes 2. DAXX pathways :the excitation of the apoptosis from TGFb by the phosphorylation of the DAXX protein ,with the hipk2 and the ask1 to activates the MAP2K3 ,with p53 ,ands to the apoptosis( the pathway 1) ,while in the another pathway ( the pathway 2 ) which did not depend on the P53 only from the MAPK8 ,ends to the apoptosis . From these two examples , we find in the proteins balances ,different types of the proteins needed to continue the cascades ,it means , the array of the proteins depends on the different types with the different charges ,so the balances in the protein cascades very important to achieve the targets , so we can get an important benefits in our researches . 3. in the preparing the cancer cells for the apoptosis ,we uses the switch proteins to oblige the cancer cells for the apoptosis , with the uses of the different types of the proteins as before ,these deferent types of the proteins has different types of the residues( the P53 has rich prolin in its domain ,may be suitable for many residues and not suitable for the others ), and in its end sides different types of the charges, so the attractive and incongruity forces plays an important roles in the array of the proteins in the cascades of the apoptosis , and to solve this problems we must do: a. we uses small series of the amino acids or very short proteins with different amino acids in its ends to fill the gaps between the proteins cascades and to modify the charges , to oblige the cancer cell for the apoptosis ,by cutting the long series of the proteins by the proteases enzymes or different types of the kallekrien. b. by the uses of the multi artificial of the short series of the nucleic acid of the messenger RNA to form these short proteins with normal ways . c. we can use the amino acids with isotopes to isolate and recognize the active types of the short proteins ( the structure sand the residues) . d .we use these types of the super proteins ( the links proteins ) with great amounts and with different randomly lengths, to get more benefits in the filling the electromagnetic gaps between the proteins in the cascades to discover the active types 4.one of the most important steps, for the successful for the apoptosis, the uses of the proteins : a. the Cro proteins which formed from the viruses for the lytic cells for the host cells ,so we can use these types of the proteins beside the other steps before, for the cancer cells. b. we can use the proteins which formed from the retro viruses ,the Tat types which activates the formation or the activation the TRAIL which mediates the apoptosis . so we can activate the cancer cells for the apoptosis and the damages by these proteins which participate for the apoptosis by the normal ways without the uses of the vital organisms .
Posted on: Fri, 21 Nov 2014 18:42:35 +0000
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