We have developed a PCR method, coined Déjà vu PCR, that - TopicsExpress

We have developed a PCR method, coined Déjà vu PCR, that utilizes six nucleotides in PCR with two methyl specific restriction enzymes that respectively digest these additional nucleotides. Use of this enzyme-and-nucleotide combination enables what we term a “DNA diode”, where DNA can advance in a laboratory in only one direction and cannot feedback into upstream assays. These additional nucleotides enable a novel DNA decontamination technique that generates ephemeral and easy to decontaminate DNA. plosone.org/article/info:doi/10.1371/journal.pone.0096492

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