Indian Journal of Agricultural Research article published in Volume 47 Issue 4 (August 2013):- HEAT STRESS ASSOCIATED ANTIOXIDANT ISOENZYMES IN WHEAT: EXPRESSION AND PROTEOMICS Ranjeet R. Kumar*, Suneha Goswami, Sushil K. Sharma, Kritika A. Gadpayle, Khushboo Singh, Narender Kumar, Gyanendra K. Rai1 and Raj D. Rai Division of Biochemistry, Indian Agricultural Research Institute, New Delhi-110 012, India Received: 06-09-2012 Accepted: 08-10-2013 ABSTRACT Abiotic stress leads to change in the expression and activities of number of proteins which are involved in defense mechanism against stresses. Qualitative and quantitative changes in proteins might play a role in signal transduction, antioxidative defence, heat shock, metal binding or osmolyte synthesis. Here, 22 wheat genotypes were screened for its cell membrane stability (CMS), out of which C-306 showed the maximum CMS value of 74%. Protein profiling revealed the expression of many new and existing proteins in C-306 cultivar compared to PBW343 under differential heat shock (HS). An altered protein expression was also observed in tolerant and susceptible cultivars at different stages of growth. Isoenzymic profiling revealed the expression of many new isoenzymes of ascorbate peroxidase and superoxide dismutase in C-306 compared to PBW343 in response to heat shock. A stage specific analysis showed the maximum activity of different antioxidant isoenzymes at pollination and seed hardening stages. SODs plays central role in regulating defense mechanism and need to be further characterized. Key words: Antioxidant enzymes, Heat shock protein, Heat stress, Isoenzymes, Protein profiling. Source: arccjournals/current-issue/821.html
Posted on: Sat, 17 Aug 2013 09:24:36 +0000
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