Review for 1061 sections 1, 2, 3 (Chris) These are practice questions (try to answer these without notes or book). Not everything is here, but if you can answer these easily then you are doing well. Pre-midterm: What is the BSL of this lab? Where do you throw gloves away when you are done with them? What is the resolving power of a phase-contrast microscope? Define aseptically. What is the advantage to using the four-quadrant streak method over the spread plate method? The counterstain of the Gram stain is ___________. A basic stain has a _____ charge, and a negative stain has a _______ charge. What types of organisms is a negative stain used for? List all of the general colony characteristics that you could observe on any isolated colony. What is the pH indicator in the MSA plate? What is the selective ingredient in the MSA plate? Is the CNA plate selective for Gram positive or Gram negative bacteria? What color does and organism appear on the HEA plate if it reduces sulfur? Describe the appearance of a positive catalase test. Which tool do you use for the oxidase test? Phenol red is what color when the tube is acidic (low pH)? Which reagents are added to the VP test? Which reagents are added to the nitrate test? Which inoculating tool did you use for the citrate test? Post-midterm: What is the differential indicator in the BEA medium? Which type of Streptococcus can you identify with the CAMP test? What is the aerotolerance if the organism grows only at the top of the FT medium? What is the cap color on the FT medium? Which four incubators did you use in the temperature experiment? What is the differential ingredient in the SIM tube for the indole test? Which carbohydrates are present in the TSI slant? What is the mechanism of action of Vancomycin? What is the name of the inoculation method you used to determine antibiotic susceptibility? To solve the Single Unknown, you used a _________ to see the test results for all of the unknown organisms. Which fungal class does Rhizopus belong to? How are protozoans classified? Which nematode was described in class?
Posted on: Thu, 24 Apr 2014 22:53:18 +0000
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